This study was designcd to investigate the effect of estrogen(Est) on the progestcrone(Prog) target cells by autoradiography. The spayed 16 mice(ICR, approximately 18∼25g) were randomly alloted into 3 groups. ³H-Prog-treated group were injected with 40 μCi of ³H-Prog/mouse/day for 1 day, Est + ³H-Prog-treated group with 20 μCi of 17 β-Est/mouse/day for 3 days and then with 40 μCi of ³H-Prog/mouse at 4th day, and Est+³H-thymidine(TdR)-treated group with 20㎍ of 17 β-Est/mouse/day for 3 days and then 80μCi of ³H-TdR/mouse at 4th days.
1. Mice uteri of both Est+³H-Prog-treated group and Est+³H-TdR-treated group were hypemophied in gross finding and the endometrium and myometrium were thickened in microscopic findings. These findings were confirmed that Est enlarged the uteri of mice.
2. Cryo-preparations of mice organs were processed for autoradiography using Kodak NTB-2 emulsion following Kodak D-19 developer and hematoxylin counterstain. In each group, the number values of silver grain distribution appeared to be higher in the ³H-Prog-treated group than in the Est+³H-Prog-treated group. It was considered that Est and Prog inhibit each other in action.
3. In both ³H-Prog-treated group and Est+³H-Prog-treated group, the uteri have highest distribution rates of silver grains than in other organs, and the cerebral neurons, hepatocytes, bronchiolar epithelial cells and splenic reticular cells also contained some silver grains.
4. The orders of the cell types with more number of silver grains in the uteri were stromal cells, glandular epithelial cells, luminal surface cells and muscular cells and also were as above orders in distribution of proliferating cell type by ³H-TdR.
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